John Lednicky

John Lednicky

Research Professor

Department: HP-ENVIRONMENTAL GLOBAL HLTH
Business Phone: (352) 273-9204
Business Email: jlednicky@phhp.ufl.edu

About John Lednicky

John Lednicky has BS, MS, and PhD degrees in Microbiology. After graduation with a BS degree, he worked as a clinical microbiology technician and attained two professional certifications: M(ASCP) and RM(NRCM). For both certifications, his specialty areas were in bacteriology and mycology. Thereafter, he worked with Staphylococcus aureus for his MS degree, and had planned on pursuing a PhD in biochemical physics. Instead, he studied transcription regulation by transcription factor SP1 in a macaque polyomavirus (SV40) model for his PhD work.

Prior to joining the University of Florida, Dr. Lednicky was an assistant professor of pathology at the Loyola Medical Center in Illinois. He then worked in industry, where he engaged in biodefense-related work, aerobiology related to inhalation exposure studies in small animal models, various projects with avian influenza H5N1 and other influenza viruses, and the production of biodiesel from alga.

Dr. Lednicky’s current research focus areas at UF are: (a) aerovirology, (b) virus discovery, (c) virus surveillance with emphasis on arthropod-borne viruses, and (d) influenza virus studies. His laboratory discovered Rhinovirus C-51 and a unique variant of Human polyomavirus 9, and was the first to detect Zika and Mayaro viruses in Haitians. More recently, his laboratory was the first in the world to discover Madariaga and Keystone viruses in humans. His laboratory is also involved with the isolation of viruses that are causing lethal infections in farmed deer of Florida. Since January of 2020, a major thrust has been work related to COVID-19. His laboratory is isolating SARS-CoV-2 from humans and the environment, and he has posted a few full-genome sequences of these viruses in GenBank.

Research Profile

Dr. John Lednicky’s current research interests include the detection, isolation, and genetic analyses of influenza viruses (both human and animal), live agent bioaerosol inhalation studies, the detection, isolation and sequence analyses of respiratory viruses, polyomavirus regulatory region structure and function, molecular and classical diagnostic virology, development of human and animal virus detection, isolation and identification methodologies, and reverse genetics of RNA viruses.

Publications

2021
Coronavirus 2019 Infectious Disease Epidemic: Where We Are, What Can Be Done and Hope For.
Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer. 16(4):546-571 [DOI] 10.1016/j.jtho.2020.12.014. [PMID] 33422679.
2021
Earliest detection to date of SARS-CoV-2 in Florida: Identification together with influenza virus on the main entry door of a university building, February 2020.
PloS one. 16(1) [DOI] 10.1371/journal.pone.0245352. [PMID] 33439885.
2021
In-Frame 12-Nucleotide Deletion within Open Reading Frame 3a in a SARS-CoV-2 Strain Isolated from a Patient Hospitalized with COVID-19.
Microbiology resource announcements. 10(8) [DOI] 10.1128/MRA.00137-21. [PMID] 33632859.
2021
SARS-CoV-2 Positivity on or After 9 Days Among Quarantined Student Contacts of Confirmed Cases.
JAMA. [DOI] 10.1001/jama.2021.2392. [PMID] 33605978.
2021
Unaltered influenza disease outcomes in swine prophylactically treated with α-galactosylceramide.
Developmental and comparative immunology. 114 [DOI] 10.1016/j.dci.2020.103843. [PMID] 32871161.
2020
A practical and economic approach for assessing potential SARS-CoV-2 transmission risk in COVID-19 patients.
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America. [DOI] 10.1093/cid/ciaa1789. [PMID] 33258915.
2020
Central nervous system involvement by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Journal of medical virology. 92(7):699-702 [DOI] 10.1002/jmv.25915. [PMID] 32314810.
2020
Collection of SARS-CoV-2 Virus from the Air of a Clinic Within a University Student Health Care Center and Analyses of the Viral Genomic Sequence.
Aerosol and air quality research. 20(6):1167-1171 [DOI] 10.4209/aaqr.2020.02.0202. [PMID] 33424954.
2020
Coronavirus Disease 2019 (COVID-19) and pregnancy: what obstetricians need to know.
American journal of obstetrics and gynecology. 222(5):415-426 [DOI] 10.1016/j.ajog.2020.02.017. [PMID] 32105680.
2020
Coronaviruses: Facts, Myths, and Hypotheses.
Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer. 15(5):675-678 [DOI] 10.1016/j.jtho.2020.02.024. [PMID] 32151778.
2020
Environmental and Aerosolized Severe Acute Respiratory Syndrome Coronavirus 2 Among Hospitalized Coronavirus Disease 2019 Patients.
The Journal of infectious diseases. 222(11):1798-1806 [DOI] 10.1093/infdis/jiaa575. [PMID] 32905595.
2020
Genome Sequence of a CHeRI Orbivirus 3 Strain Isolated from a Dead White-Tailed Deer (Odocoileus virginianus) in Florida, USA.
Microbiology resource announcements. 9(26) [DOI] 10.1128/MRA.00523-20. [PMID] 32586868.
2020
Hydroxyl functionalized multi-walled carbon nanotubes modulate immune responses without increasing 2009 pandemic influenza A/H1N1 virus titers in infected mice.
Toxicology and applied pharmacology. 404 [DOI] 10.1016/j.taap.2020.115167. [PMID] 32771490.
2020
Panspecies molecular assays detect viral pathogens missed by real-time PCR/reverse-transcriptase PCR among pneumonia patients, Sarawak, Malaysia.
Tropical diseases, travel medicine and vaccines. 6 [DOI] 10.1186/s40794-020-00114-2. [PMID] 32817802.
2020
Under-the-Radar Dengue Virus Infections in Natural Populations of Aedes aegypti Mosquitoes.
mSphere. 5(2) [DOI] 10.1128/mSphere.00316-20. [PMID] 32350095.
2020
Viable SARS-CoV-2 in the air of a hospital room with COVID-19 patients.
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases. 100:476-482 [DOI] 10.1016/j.ijid.2020.09.025. [PMID] 32949774.
2020
Viable SARS-CoV-2 in the air of a hospital room with COVID-19 patients.
medRxiv : the preprint server for health sciences. [DOI] 10.1101/2020.08.03.20167395. [PMID] 32793914.
2019
Characterization of mule deerpox virus in Florida white-tailed deer fawns expands the known host and geographic range of this emerging pathogen.
Archives of virology. 164(1):51-61 [DOI] 10.1007/s00705-018-3991-7. [PMID] 30238163.
2019
Clinical and Epidemiologic Patterns of Chikungunya Virus Infection and Coincident Arboviral Disease in a School Cohort in Haiti, 2014-2015.
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America. 68(6):919-926 [DOI] 10.1093/cid/ciy582. [PMID] 30184178.
2019
Collection, particle sizing and detection of airborne viruses.
Journal of applied microbiology. 127(6):1596-1611 [DOI] 10.1111/jam.14278. [PMID] 30974505.
2019
Condensational particle growth device for reliable cell exposure at the air-liquid interface to nanoparticles.
Aerosol science and technology : the journal of the American Association for Aerosol Research. 53(12):1415-1428 [DOI] 10.1080/02786826.2019.1659938. [PMID] 33033421.
2019
Cutaneous features of Zika virus infection: a clinicopathological overview.
Clinical and experimental dermatology. 44(1):13-19 [DOI] 10.1111/ced.13793. [PMID] 30267436.
2019
Determination of the distribution of infectious viruses in aerosol particles using water-based condensational growth technology and a bacteriophage MS2 model.
Aerosol science and technology : the journal of the American Association for Aerosol Research. 53(5):583-593 [DOI] 10.1080/02786826.2019.1581917. [PMID] 31359905.
2019
Emergence of Madariaga virus as a cause of acute febrile illness in children, Haiti, 2015-2016.
PLoS neglected tropical diseases. 13(1) [DOI] 10.1371/journal.pntd.0006972. [PMID] 30629592.
2019
Genome Sequences of a Novel Strain of Big Cypress Orbivirus Isolated from a Dead Florida White-Tailed Deer (Odocoileus virginianus).
Microbiology resource announcements. 8(11) [DOI] 10.1128/MRA.01717-18. [PMID] 30938331.
2019
Isolation of Mayaro Virus from a Venezuelan Patient with Febrile Illness, Arthralgias, and Rash: Further Evidence of Regional Strain Circulation and Possible Long-Term Endemicity.
The American journal of tropical medicine and hygiene. 101(6):1219-1225 [DOI] 10.4269/ajtmh.19-0357. [PMID] 31595869.
2019
Keystone Virus Isolated From a Florida Teenager With Rash and Subjective Fever: Another Endemic Arbovirus in the Southeastern United States?
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America. 68(1):143-145 [DOI] 10.1093/cid/ciy485. [PMID] 29893806.
2019
Mayaro as a Caribbean traveler: Evidence for multiple introductions and transmission of the virus into Haiti.
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases. 87:151-153 [DOI] 10.1016/j.ijid.2019.07.031. [PMID] 31382049.
2019
Molecular characterization of a novel reassortment Mammalian orthoreovirus type 2 isolated from a Florida white-tailed deer fawn.
Virus research. 270 [DOI] 10.1016/j.virusres.2019.197642. [PMID] 31228510.
2019
Resurgence of Vaccine-Preventable Diseases in Venezuela as a Regional Public Health Threat in the Americas.
Emerging infectious diseases. 25(4):625-632 [DOI] 10.3201/eid2504.181305. [PMID] 30698523.
2019
Single-walled carbon nanotubes repress viral-induced defense pathways through oxidative stress.
Nanotoxicology. 13(9):1176-1196 [DOI] 10.1080/17435390.2019.1645903. [PMID] 31328592.
2019
Three New Orbivirus Species Isolated from Farmed White-Tailed Deer (Odocoileus virginianus) in the United States.
Viruses. 12(1) [DOI] 10.3390/v12010013. [PMID] 31861885.
2019
Venezuela’s humanitarian crisis, resurgence of vector-borne diseases, and implications for spillover in the region.
The Lancet. Infectious diseases. 19(5):e149-e161 [DOI] 10.1016/S1473-3099(18)30757-6. [PMID] 30799251.
2018
A new “American” subgroup of African-lineage Chikungunya virus detected in and isolated from mosquitoes collected in Haiti, 2016.
PloS one. 13(5) [DOI] 10.1371/journal.pone.0196857. [PMID] 29746539.
2018
An efficient virus aerosol sampler enabled by adiabatic expansion.
Journal of aerosol science. 117:74-84 [DOI] 10.1016/j.jaerosci.2018.01.001. [PMID] 32226117.
2018
Collection of Airborne Bacteria and Yeast Through Water-Based Condensational Growth
Aerobiologia. 34(3):337-348 [DOI] 10.1007/s10453-018-9517-7.
2018
Complete Genome Sequence of Dengue Virus Serotype 2, Asian/American Genotype, Isolated from the Urine of a Venezuelan Child with Hemorrhagic Fever in 2016.
Genome announcements. 6(24) [DOI] 10.1128/genomeA.00529-18. [PMID] 29903824.
2018
Complete Genome Sequence of Epizootic hemorrhagic disease virus Serotype 6, Isolated from Florida White-Tailed Deer (Odocoileus virginianus).
Genome announcements. 6(14) [DOI] 10.1128/genomeA.00160-18. [PMID] 29622607.
2018
Detection and phylogenetic characterization of arbovirus dual-infections among persons during a chikungunya fever outbreak, Haiti 2014.
PLoS neglected tropical diseases. 12(5) [DOI] 10.1371/journal.pntd.0006505. [PMID] 29851952.
2018
Evidence for Cross-species Influenza A Virus Transmission Within Swine Farms, China: A One Health, Prospective Cohort Study.
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America. 66(4):533-540 [DOI] 10.1093/cid/cix823. [PMID] 29401271.
2018
Evidence for Mother-to-Child Transmission of Zika Virus Through Breast Milk.
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America. 66(7):1120-1121 [DOI] 10.1093/cid/cix968. [PMID] 29300859.
2018
Evidence of Chikungunya Virus Disease in Pakistan Since 2015 With Patients Demonstrating Involvement of the Central Nervous System.
Frontiers in public health. 6 [DOI] 10.3389/fpubh.2018.00186. [PMID] 30042937.
2018
Human West Nile Virus Disease Outbreak in Pakistan, 2015-2016.
Frontiers in public health. 6 [DOI] 10.3389/fpubh.2018.00020. [PMID] 29535994.
2018
Isolation and identification of human coronavirus 229E from frequently touched environmental surfaces of a university classroom that is cleaned daily.
American journal of infection control. 46(1):105-107 [DOI] 10.1016/j.ajic.2017.07.014. [PMID] 28893443.
2018
Madariaga Virus: Identification of a Lineage III Strain in a Venezuelan Child With Acute Undifferentiated Febrile Illness, in the Setting of a Possible Equine Epizootic.
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America. 67(4):619-621 [DOI] 10.1093/cid/ciy224. [PMID] 29718127.
2018
Spondweni Virus in Field-Caught Culex quinquefasciatus Mosquitoes, Haiti, 2016.
Emerging infectious diseases. 24(9):1765-1767 [DOI] 10.3201/eid2409.171957. [PMID] 30124422.
2018
Valve-Enabled Sample Preparation and RNA Amplification in a Coffee Mug for Zika Virus Detection.
Angewandte Chemie (International ed. in English). 57(52):17211-17214 [DOI] 10.1002/anie.201809993. [PMID] 30358036.
2017
A Role of Sp1 Binding Motifs in Basal and Large T-Antigen-Induced Promoter Activities of Human Polyomavirus HPyV9 and Its Variant UF-1.
International journal of molecular sciences. 18(11) [DOI] 10.3390/ijms18112414. [PMID] 29135936.
2017
Aerosolized avian influenza A (H5N6) virus isolated from a live poultry market, China.
The Journal of infection. 74(1):89-91 [DOI] 10.1016/j.jinf.2016.08.002. [PMID] 27519618.
2017
Collection of Viable Aerosolized Influenza Virus and Other Respiratory Viruses in a Student Health Care Center through Water-Based Condensation Growth.
mSphere. 2(5) [DOI] 10.1128/mSphere.00251-17. [PMID] 29034325.
2017
Complete Genome Sequence of Dengue virus Type 2 from a Resident of North-Central Florida with Locally Transmitted Dengue Fever.
Genome announcements. 5(31) [DOI] 10.1128/genomeA.00782-17. [PMID] 28774993.
2017
Complete Genome Sequence of Human Coronavirus Strain 229E Isolated from Plasma Collected from a Haitian Child in 2016.
Genome announcements. 5(47) [DOI] 10.1128/genomeA.01313-17. [PMID] 29167251.
2017
Complete Genome Sequences of Chikungunya Viruses Isolated from Plasma Specimens Collected from Haitians in 2014.
Genome announcements. 5(15) [DOI] 10.1128/genomeA.00148-17. [PMID] 28408671.
2017
Complete Genome Sequences of Identical Zika virus Isolates in a Nursing Mother and Her Infant.
Genome announcements. 5(17) [DOI] 10.1128/genomeA.00231-17. [PMID] 28450510.
2017
Complete Genomic Sequence of Dengue virus 1, Isolated from Plasma Collected from a Haitian Child in 2014.
Genome announcements. 5(22) [DOI] 10.1128/genomeA.00331-17. [PMID] 28572304.
2017
Detection of Alphacoronavirus vRNA in the Feces of Brazilian Free-Tailed Bats (Tadarida brasiliensis) from a Colony in Florida, USA.
Diseases (Basel, Switzerland). 5(1) [DOI] 10.3390/diseases5010007. [PMID] 28933360.
2017
Drifted Influenza A and B Viruses Collected by a Water-Based Condensation Growth Air Sampler in a Student Health Care Center during an Influenza Outbreak.
Genome announcements. 5(15) [DOI] 10.1128/genomeA.00178-17. [PMID] 28408687.
2017
Emergence of recombinant Mayaro virus strains from the Amazon basin.
Scientific reports. 7(1) [DOI] 10.1038/s41598-017-07152-5. [PMID] 28821712.
2017
Genomic Sequences of Epizootic Hemorrhagic Disease Viruses Isolated from Florida White-Tailed Deer.
Genome announcements. 5(43) [DOI] 10.1128/genomeA.01174-17. [PMID] 29074661.
2017
Isolation of Coronavirus NL63 from Blood from Children in Rural Haiti: Phylogenetic Similarities with Recent Isolates from Malaysia.
The American journal of tropical medicine and hygiene. 96(1):144-147 [DOI] 10.4269/ajtmh.16-0585. [PMID] 27799635.
2017
Overcoming qRT-PCR interference by select carbon nanotubes in assessments of gene expression.
BioTechniques. 63(2):81-84 [DOI] 10.2144/000114578. [PMID] 28803544.
2017
Semi-quantitative Influenza A population averages from a multiplex respiratory viral panel (RVP): potential for reflecting target sequence changes affecting the assay.
Virology journal. 14(1) [DOI] 10.1186/s12985-017-0796-3. [PMID] 28709460.
2017
Single-walled carbon nanotubes modulate pulmonary immune responses and increase pandemic influenza a virus titers in mice.
Virology journal. 14(1) [DOI] 10.1186/s12985-017-0909-z. [PMID] 29273069.
2017
The Use of Bioaerosol Sampling for Airborne Virus Surveillance in Swine Production Facilities: A Mini Review.
Frontiers in veterinary science. 4 [DOI] 10.3389/fvets.2017.00121. [PMID] 28798919.
2016
AOAC SMPR 2015.011.
Journal of AOAC International. 99(1):298-302 [DOI] 10.5740/jaoac.int.SMPR2015.011. [PMID] 28482998.
2016
Bioaerosol Sampling in Modern Agriculture: A Novel Approach for Emerging Pathogen Surveillance?
The Journal of infectious diseases. 214(4):537-45 [DOI] 10.1093/infdis/jiw180. [PMID] 27190187.
2016
COINFECTION OF CALIFORNIA SEA LION ADENOVIRUS 1 AND A NOVEL POLYOMAVIRUS IN A HAWAIIAN MONK SEAL (NEOMONACHUS SCHAUINSLANDI).
Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians. 47(2):427-37 [DOI] 10.1638/2014-0252.1. [PMID] 27468013.
2016
Complete Genome Sequence of Enterovirus D68 Detected in Classroom Air and on Environmental Surfaces.
Genome announcements. 4(3) [DOI] 10.1128/genomeA.00579-16. [PMID] 27313311.
2016
Efficient collection of viable virus aerosol through laminar-flow, water-based condensational particle growth.
Journal of applied microbiology. 120(3):805-15 [DOI] 10.1111/jam.13051. [PMID] 26751045.
2016
Flaviviruses as a Cause of Undifferentiated Fever in Sindh Province, Pakistan: A Preliminary Report.
Frontiers in public health. 4 [DOI] 10.3389/fpubh.2016.00008. [PMID] 26909342.
2016
Hemagglutinin Gene Clade 3C.2a Influenza A(H3N2) Viruses, Alachua County, Florida, USA, 2014-15.
Emerging infectious diseases. 22(1):121-3 [DOI] 10.3201/2201.151019. [PMID] 26692074.
2016
Highly Efficient Collection of Infectious Pandemic Influenza H1N1 Virus (2009) Through Laminar-Flow Water Based Condensation
Aerosol Science and Technology. 50(7):I-IV [DOI] 10.1080/02786826.2016.1179254.
2016
Isolation of an Enterovirus D68 from Blood from a Child with Pneumonia in Rural Haiti: Close Phylogenetic Linkage with New York Strain.
The Pediatric infectious disease journal. 35(9):1048-50 [DOI] 10.1097/INF.0000000000001283. [PMID] 27331858.
2016
Mayaro Virus in Child with Acute Febrile Illness, Haiti, 2015.
Emerging infectious diseases. 22(11):2000-2002 [DOI] 10.3201/eid2211.161015. [PMID] 27767924.
2016
Rapid control of pandemic H1N1 influenza by targeting NKT-cells.
Scientific reports. 6 [DOI] 10.1038/srep37999. [PMID] 27897246.
2016
Serologic evidence of exposure to influenza D virus among persons with occupational contact with cattle.
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology. 81:31-3 [DOI] 10.1016/j.jcv.2016.05.017. [PMID] 27294672.
2016
Use of RNA amplification and electrophoresis for studying virus aerosol collection efficiency and their comparison with plaque assays.
Electrophoresis. 37(19):2574-2580 [DOI] 10.1002/elps.201600141. [PMID] 27196379.
2016
Zika and Chikungunya virus co-infection in a traveller returning from Colombia, 2016: virus isolation and genetic analysis.
JMM case reports. 3(6) [DOI] 10.1099/jmmcr.0.005072. [PMID] 28348794.
2016
Zika Virus Outbreak in Haiti in 2014: Molecular and Clinical Data.
PLoS neglected tropical diseases. 10(4) [DOI] 10.1371/journal.pntd.0004687. [PMID] 27111294.
2016
α-Galactosylceramide protects swine against influenza infection when administered as a vaccine adjuvant.
Scientific reports. 6 [DOI] 10.1038/srep23593. [PMID] 27004737.
2015
Public health education at the University of Florida: synergism and educational innovation.
American journal of public health. 105 Suppl 1:S83-7 [DOI] 10.2105/AJPH.2014.302414. [PMID] 25706027.
2015
Severity of influenza A(H1N1) illness and emergence of D225G variant, 2013-14 influenza season, Florida, USA.
Emerging infectious diseases. 21(4):664-7 [DOI] 10.3201/eid2104.141375. [PMID] 25811540.
2014
Complete genomic sequence of a new Human polyomavirus 9 strain with an altered noncoding control region.
Virus genes. 49(3):490-2 [DOI] 10.1007/s11262-014-1119-z. [PMID] 25260554.
2014
Environmental sampling for respiratory pathogens in Jeddah airport during the 2013 Hajj season.
American journal of infection control. 42(12):1266-9 [DOI] 10.1016/j.ajic.2014.07.027. [PMID] 25465254.
2014
Isolation of Tacaribe virus, a Caribbean arenavirus, from host-seeking Amblyomma americanum ticks in Florida.
PloS one. 9(12) [DOI] 10.1371/journal.pone.0115769. [PMID] 25536075.
2014
Single-walled carbon nanotubes increase pandemic influenza A H1N1 virus infectivity of lung epithelial cells.
Particle and fibre toxicology. 11 [DOI] 10.1186/s12989-014-0066-0. [PMID] 25497303.
2013
Detection and Isolation of Airborne Influenza A H3N2 Virus Using a Sioutas Personal Cascade Impactor Sampler.
Influenza research and treatment. 2013 [DOI] 10.1155/2013/656825. [PMID] 24224087.
2013
Isolation and genetic characterization of human coronavirus NL63 in primary human renal proximal tubular epithelial cells obtained from a commercial supplier, and confirmation of its replication in two different types of human primary kidney cells.
Virology journal. 10 [DOI] 10.1186/1743-422X-10-213. [PMID] 23805916.
2012
Comparative Analysis of the Full-Length Genome Sequence of a Clinical Isolate of Human Parainfluenza Virus 4B.
Scientifica. 2012 [DOI] 10.6064/2012/871201. [PMID] 24278751.
2011
Higher titers of some H5N1 and recent human H1N1 and H3N2 influenza viruses in Mv1 Lu vs. MDCK cells.
Virology journal. 8 [DOI] 10.1186/1743-422X-8-66. [PMID] 21314955.
2010
A nonlethal young domesticated ferret (Mustela putorius furo) model for studying pandemic influenza virus A/California/04/2009 (H1N1).
Comparative medicine. 60(5):364-8 [PMID] 21262121.
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2010
Design, assembly, and validation of a nose-only inhalation exposure system for studies of aerosolized viable influenza H5N1 virus in ferrets.
Virology journal. 7 [DOI] 10.1186/1743-422X-7-135. [PMID] 20573226.
2010
Ferrets develop fatal influenza after inhaling small particle aerosols of highly pathogenic avian influenza virus A/Vietnam/1203/2004 (H5N1).
Virology journal. 7 [DOI] 10.1186/1743-422X-7-231. [PMID] 20843329.
2010
Gas-permeable ethylene bags for the small scale cultivation of highly pathogenic avian influenza H5N1 and other viruses in embryonated chicken eggs.
Virology journal. 7 [DOI] 10.1186/1743-422X-7-23. [PMID] 20109234.
2010
Pandemic preparedness & the development of vaccines for H5N1 influenza at the Midwest Research Institute.
Missouri medicine. 107(5):298-301 [PMID] 21207777.
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2010
Validation of a method for preparing influenza H5N1 simulated samples.
Journal of virological methods. 167(2):125-31 [DOI] 10.1016/j.jviromet.2010.03.022. [PMID] 20362615.
2006
Uncommon respiratory pathogens.
Current opinion in pulmonary medicine. 12(3):235-9 [PMID] 16582680.
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2005
Some oral poliovirus vaccines were contaminated with infectious SV40 after 1961.
Cancer research. 65(22):10273-9 [PMID] 16288015.
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2004
Effective primary isolation of wild-type canine distemper virus in MDCK, MV1 Lu and Vero cells without nucleotide sequence changes within the entire haemagglutinin protein gene and in subgenomic sections of the fusion and phospho protein genes.
Journal of virological methods. 118(2):147-57 [PMID] 15081610.
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2004
Fatal hemorrhagic pneumonia concomitant with Chlamydia pneumoniae and parainfluenza virus 4 infection.
Archives of pathology & laboratory medicine. 128(6):640-4 [PMID] 15163237.
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2004
Genetically distant American Canine distemper virus lineages have recently caused epizootics with somewhat different characteristics in raccoons living around a large suburban zoo in the USA.
Virology journal. 1 [PMID] 15507154.
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2004
Molecular analysis of SV-40-CAL, a new slow growing SV-40 strain from the kidney of a caged New World monkey with fatal renal disease.
Virus genes. 29(2):183-90 [PMID] 15284478.
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2004
Phylogenetic analysis of polyomavirus simian virus 40 from monkeys and humans reveals genetic variation.
Journal of virology. 78(17):9306-16 [PMID] 15308725.
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2004
Reverse transcription-polymerase chain reaction detection and nucleic acid sequence confirmation of reovirus infection in laboratory mice with discordant serologic indirect immunofluorescence assay and enzyme-linked immunosorbent assay results.
Comparative medicine. 54(4):410-7 [PMID] 15357322.
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2003
Hantaviruses. a short review.
Archives of pathology & laboratory medicine. 127(1):30-5 [PMID] 12521363.
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2003
Polyomavirus JCV excretion and genotype analysis in HIV-infected patients receiving highly active antiretroviral therapy.
AIDS (London, England). 17(6):801-7 [PMID] 12660526.
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2003
SV40 and human brain tumors.
International journal of cancer. 106(1):140-2; author reply 143 [PMID] 12794770.
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2003
The dynamics of herpesvirus and polyomavirus reactivation and shedding in healthy adults: a 14-month longitudinal study.
The Journal of infectious diseases. 187(10):1571-80 [PMID] 12721937.
View on: PubMed
2002
Detection of polyomavirus simian virus 40 tumor antigen DNA in AIDS-related systemic non-Hodgkin lymphoma.
Journal of acquired immune deficiency syndromes (1999). 29(2):109-16 [PMID] 11832678.
View on: PubMed
2002
PCR detection and DNA sequence analysis of the regulatory region of lymphotropic papovavirus in peripheral blood mononuclear cells of an immunocompromised rhesus macaque.
Journal of clinical microbiology. 40(3):1056-9 [PMID] 11880438.
View on: PubMed
2001
Detection of SV40 DNA sequences in human tissue.
Methods in molecular biology (Clifton, N.J.). 165:257-67 [PMID] 11217392.
View on: PubMed
2001
Simian virus 40 regulatory region structural diversity and the association of viral archetypal regulatory regions with human brain tumors.
Seminars in cancer biology. 11(1):39-47 [PMID] 11243898.
View on: PubMed
2000
Molecular characterization of SV40 DNA in multiple samples from a human mesothelioma.
Anticancer research. 20(2A):879-84 [PMID] 10810370.
View on: PubMed
2000
Response to more about: cell and molecular biology of simian virus 40: implications for human infections and disease.
Journal of the National Cancer Institute. 92(6):496-7 [PMID] 10716972.
View on: PubMed
1999
Molecular evidence of simian virus 40 infections in children.
The Journal of infectious diseases. 180(3):884-7 [PMID] 10438386.
View on: PubMed
1999
Polyomaviruses and human tumors: a brief review of current concepts and interpretations.
Frontiers in bioscience : a journal and virtual library. 4:D153-64 [PMID] 9989950.
View on: PubMed
1998
Consideration of PCR methods for the detection of SV40 in tissue and DNA specimens.
Developments in biological standardization. 94:155-64 [PMID] 9776238.
View on: PubMed
1998
Cytotoxic T lymphocyte recognition sequences as markers for distinguishing among tumour antigens encoded by SV40, BKV and JCV.
Developments in biological standardization. 94:329-39 [PMID] 9776254.
View on: PubMed
1998
Detection of authentic SV40 DNA sequences in human brain and bone tumours.
Developments in biological standardization. 94:23-32 [PMID] 9776222.
View on: PubMed
1998
Natural isolates of simian virus 40 from immunocompromised monkeys display extensive genetic heterogeneity: new implications for polyomavirus disease.
Journal of virology. 72(5):3980-90 [PMID] 9557685.
View on: PubMed
1998
Sequence analyses of human tumor-associated SV40 DNAs and SV40 viral isolates from monkeys and humans.
Journal of neurovirology. 4(2):182-93 [PMID] 9584955.
View on: PubMed
1997
A coupled PCR and restriction digest method for the detection and analysis of the SV40 regulatory region in infected-cell lysates and clinical samples.
Journal of virological methods. 64(1):1-9 [PMID] 9029524.
View on: PubMed
1997
High-fidelity PCR amplification of infectious copies of the complete simian virus 40 genome from plasmids and virus-infected cell lysates.
Gene. 184(2):189-95 [PMID] 9031627.
View on: PubMed
1997
Loss of T-antigen sequences allows SV40-transformed human cells in crisis to acquire a senescent-like phenotype.
The journals of gerontology. Series A, Biological sciences and medical sciences. 52(5):B229-34 [PMID] 9310070.
View on: PubMed
1997
SV40 and human brain tumors.
Journal of neurovirology. 3 Suppl 1:S78-9 [PMID] 9179802.
View on: PubMed
1997
SV40 DNA in human osteosarcomas shows sequence variation among T-antigen genes.
International journal of cancer. 72(5):791-800 [PMID] 9311596.
View on: PubMed
1997
Tissue culture adaptation of natural isolates of simian virus 40: changes occur in viral regulatory region but not in carboxy-terminal domain of large T-antigen.
The Journal of general virology. 78 ( Pt 7):1697-705 [PMID] 9225047.
View on: PubMed
1996
Identification of a variable region at the carboxy terminus of SV40 large T-antigen.
Virology. 221(2):355-61 [PMID] 8661447.
View on: PubMed
1995
Artificial modification of the viral regulatory region improves tissue culture growth of SV40 strain 776.
Virus research. 35(2):143-53 [PMID] 7762288.
View on: PubMed
1995
Natural simian virus 40 strains are present in human choroid plexus and ependymoma tumors.
Virology. 212(2):710-7 [PMID] 7571441.
View on: PubMed
1992
Two synthetic Sp1-binding sites functionally substitute for the 21-base-pair repeat region to activate simian virus 40 growth in CV-1 cells.
Journal of virology. 66(11):6379-90 [PMID] 1328672.
View on: PubMed
1983
Isolation and characterization of a bacteriophage factor that confers competence for genetic transformation to an exfoliative toxin-producing strain of Staphylococcus aureus.
Infection and immunity. 39(2):939-47 [PMID] 6219953.
View on: PubMed

Grants

Mar 2021 ACTIVE
Multiplexed Airborne Virus Collection and Detection at the Point-of-Care
Role: Co-Investigator
Funding: NATL INST OF HLTH NIAID
Feb 2021 ACTIVE
Multiplexed Detection of Mosquito-Borne Viruses at the Point-of-Care
Role: Co-Investigator
Funding: NATL INST OF HLTH NIAID
Sep 2020 ACTIVE
Rapid disinfection using compact plasma reactors for COVID-19
Role: Principal Investigator
Funding: SURFPLASMA via NATL SCIENCE FOU
May 2020 ACTIVE
RAPID: Environmental Surveillance to Assess Aerosol Transmission Pathway of COVID-19 Enabled by On-The-Spot Sampling and Detection
Role: Co-Investigator
Funding: NATL SCIENCE FOU
May 2020 ACTIVE
RAPID: Collaborative Research: Transforming passive protective face masks toward active capture and inactivation of coronavirus with nano-assisted surfactant modification
Role: Co-Investigator
Funding: NATL SCIENCE FOU
Jan 2020 ACTIVE
EVA-GLOBAL (European Virus Archive goes Global) EU project 871029
Role: Faculty
Funding: AIX-MARSEILLE UNIVERSITY via EUROPEAN UNION
Sep 2019 – Aug 2020
Surveillance of mosquito and reservoir animal infection with Keystone virus: a possible cause of human encephalitis
Role: Co-Investigator
Funding: FL DEPT OF AG AND CON SER AG ENV SVC
Aug 2017 ACTIVE
Harnessing Natural Killer T cells to counteract swine influenza
Role: Co-Investigator
Funding: NATL INST OF HLTH NICHD
Mar 2017 – Jun 2018
Identification of antiviral therapies for the treatment of Zika virus using existing drugs
Role: Co-Investigator
Funding: FL DEPT OF HLTH
Mar 2017 – Mar 2018
Aedes mosquito surveillance and testing for host preference and prevalence of Chikungunya and dengue viruses in vector populations in Southcom AOR
Role: Co-Investigator
Funding: US DEPT OF AG AG RES SVC via US NAVY
Mar 2017 – Jun 2018
Multiplexed Detection Platform for Point-of-Service Testing of Zika Virus
Role: Co-Investigator
Funding: FL DEPT OF HLTH
Mar 2017 – Jun 2018
Rapid diagnostic test for Zika virus in dried blood spots with low demands on instrumentation
Role: Co-Investigator
Funding: FL DEPT OF HLTH
Dec 2016 ACTIVE
Southeastern Regional CoE in Vector-Borne Diseases: The Gateway Program
Role: Co-Investigator
Funding: CTRS FOR DISEASE CONTROL AND PREVENTION
Oct 2016 ACTIVE
Preparedness, research and action network on maternal-pediatric axis of ZIKV infection in Latin America and the Caribbean
Role: Co-Investigator
Funding: PENTA FOUNDATION via EUROPEAN COMMISSION
Sep 2016 – Aug 2018
Entomological Studies of Zika Virus Transmission in Haiti
Role: Co-Investigator
Funding: US DEPT OF AG NATL INST OF FOOD AND AG
Jul 2016 ACTIVE
Cholera transmission and evolution in Port-au-Prince, Haiti
Role: Project Manager
Funding: NATL INST OF HLTH NIAID
Jun 2016 – May 2019
Development of anti-Salmonella Single-chain Abs as serum diagnostics
Role: Co-Investigator
Funding: DUKE UNIV via NATL INST OF HLTH NIAID
May 2016 – Mar 2017
Aedes mosquito surveillance and testing to determine host preference and prevalence of Chikungunya and dengue viruses in vector populations in Southcom AOR
Role: Co-Investigator
Funding: US DEPT OF AG AG RES SVC
Mar 2016 – Feb 2019
Timely Risk Assessment of Airborne Virus with a Super-Efficient System for Viable Influenza Aerosol Capture
Role: Project Manager
Funding: NATL INST OF HLTH NIAID
May 2014 – Apr 2018
IDBR: Type A: Highly Efficient and Rapid Viral Aerosol Detection System (HERVADS)
Role: Project Manager
Funding: NATL SCIENCE FOU
Mar 2014 – Sep 2019
The Role of Arboviruses as a Cause of Undifferentiated Fever in Sind, Pakistan
Role: Project Manager
Funding: DEFENSE THREAT REDUCTION AGENCY
Sep 2013 – Aug 2016
Natural Killer T (NKT) Cell Control of Pandemic Swine Influenza Infection: A Novel Pig Model
Role: Project Manager
Funding: UF DSR OPPORTUNITY FUND
Jul 2012 – Apr 2018
Contribution of Toll-like Receptors in the Pulmonary Response to?Nanoparticles and Pathogens
Role: Project Manager
Funding: NATL INST OF HLTH NHLBI
Jul 2006 – Dec 2020
21st Century Research & Economic Development Investment Program (REDIP)
Role: Project Manager
Funding: STATE UNIV SYS OF FL BOARD OF GOVERNORS

Education

Doctor of Philosophy in Microbiology
1991 · University of Texas
Master of Science in Microbiology
1984 · University of Missouri
Bachelor of Science in Microbiology
1978 · University of Miami

Teaching Profile

Courses Taught
2013,2018-2021
PHC6937 Special Topics in Public Health
2014-2018,2020-2021
PHC7980 Research for Doctoral Dissertation
2012,2014-2016,2016-2020
PHC6601 Seminar in Contemporary Public Health Issues
2012-2014,2016-2020
PHC6946 Public Health Internship
2011-2021
PHC6702 Environmental Monitoring and Exposure Assessment
2015-2016,2018-2020
PHC7979 Advanced Research
2014-2018,2020
PHC6722 Environmental and Global Health Research Methods Rotation
2019
PHC6947 Occupational Health Field Research Experience
2019
PHC6945 Public Health Practicum
2018
VME7979 Advanced Research
2018
VME7980 Research for Doctoral Dissertation
2018
BME7980 Research for Doctoral Dissertation
2013-2016
PHC6036 Environmental Infectious Diseases: A Molecular Approach
2012
PHC6905 Independent Study

Contact Details

Phones:
Business:
(352) 273-9204
Emails: