John Lednicky, PhD
Office: HPNP 2146
Current Research Interests
- Collection and analyses of airborne viruses
- Avian influenza virus H5N1 and other influenza viruses (human and animal): detection, isolation, and genetic analysis
- Live agent bioaerosol inhalation studies
- Cultivation of alga and extraction of oil from alga
- Production of biodiesel from algal oils
- Respiratory viruses including hantaviruses and SARS coronavirus
- Polyomavirus (BKV, JCV, LPV, PyV, SV40) regulatory region structure and function
- Molecular and classical diagnostic virology (human and animal)
- Rodent- and lagomorph-pathogen serology and diagnostic virology
- Paramyxovirus detection, isolation, and genetic analysis, and immunogenicity of Canine distemper virus
- Development of human and animal virus detection, isolation, and identification methodologies
- Determination of whole genomic sequences of various DNA and RNA viruses
- Diagnostic (clinical) microbiology (bacteriology, mycology, parasitology, and virology)
- Reverse genetics of RNA viruses: expression of viral genes and virus production
- West Nile virus: detection, isolation, and molecular characterization
- B.S., Microbiology, University of Miami, Miami, Florida, 1978
- M.S., Microbiology, University of Missouri-Kansas City, 1984
- Ph.D., Microbiology, University of Texas-Austin, 1991, “Molecular-Genetic Analysis of the SV40 Upstream Promoter Elements”
1979. Microbiology Technician, Clinical Microbiology Laboratory, Bethany Medical Center, Kansas City, Kansas
1979-1980. Microbiology Technician, Clinical Microbiology Laboratory, Med Labs, Inc., Denton, Texas
1981-1984. Graduate Research Assistant, Department of Microbiology, University of Missouri-Kansas City
1983-1984. Laboratory Instructor, Rockhurst College, Kansas City, Missouri
1985-1989. Graduate Research and Teaching Assistant, Department of Microbiology,
(Graduate Teaching Assistant, Biology Laboratory, Department of Biology, 1984)
University of Texas–Austin
1991. Research Associate, Department of Biochemistry, (Graduate Research Assistant, Department of Biochemistry, 1989-1991)University of Missouri-Columbia
1997-2000. Research Assistant Professor, Department of Molecular Virology and Microbiology, (Postdoctoral Fellow, Department of Molecular Virology and Microbiology, 1991-1996) Baylor College of Medicine, Houston, Texas
2001-2005. Assistant Professor, Department of Pathology, Loyola University Medical Center, Maywood, Illinois
2009-2010 Senior Advisor, December 2009-September 2010 (Principal Scientist, July 2005-December 2009) Midwest Research Institute, Kansas City, Missouri
- Lednicky J., Waltzek TB, McGeehan E, Loeb JC, Hamilton SB, and Luetke MC. Isolation and genetic characterizationof human coronavirus NL63 in primary human renal proximal tubularepithelial cells obtained from a commercial supplier, and confirmation of its replication in two different types of human primary kidney cells.Virology Journal 2013 10:213
- Lednicky JA and Loeb JC. Detection and isolation of airborne Influenza A H3N2 virus using a Sioutas Personal Cascade Impactor Sampler. Influenza Research and Treatment. 10 Oct. 2013. Article ID 656825.
- John A. Lednicky and Diane E. Wyatt (2012). The Art of Animal Cell Culture for Virus Isolation, Biomedical Tissue Culture, Luca Ceccherini-Nelli and Barbara Matteoli (Ed.), ISBN: 978-953-51-0788-0, InTech
- Lednicky, J. A., T. B. Waltzek, M. D. Halpern, and S. B. Hamilton. 2012. Comparative analysis of the full-length genome sequence of a clinical isolate of Human parainfluenza virus 4B. Scientifica e871201.
- Hamilton, S. B., D. E. Wyatt, B. T. Wahlgren, M. K. O’Dowd, J. M. Morrissey, D. E. Daniels, and J. A. Lednicky. 2011. Higher titers of some H5N1 and recent human H1N1 and H3N2 influenza viruses in Mv1 Lu vs. MDCK cells. Virol. J. 8:e66.
- Lednicky, J. A., C. R. Croutch, S. J. Lawrence, S. B. Hamilton, D. E. Daniels, and A. B. Astroff. 2010. A Non-Lethal Young Domesticated Ferret (Mustela putorius furo) Model for Studying Pandemic Influenza Virus A/California/04/2009 (H1N1). Comparative Medicine. 60: 364-368.
- John A Lednicky , Sara B Hamilton , Richard S Tuttle , William A Sosna , Deirdre E Daniels and David E Swayne. “Ferrets develop fatal influenza after inhaling small particle aerosols of highly pathogenic avian influenza virus A/Vietnam/1203/2004 (H5N1)” Virology Journal 2010, 7:231doi:10.1186/1743-422X-7-231
- Lednicky, J. A., J. M.Villanueva, S. A. Burke, R. Shively, M. W. Shaw, D. E. Daniels, S. B. Hamilton, and R. O. Donis. 2010. Validation of a Method for Preparing Influenza H5N1 Simulated Samples. Journal of Virological Methods 167:125-131.
- Hamilton, S. B., D. E. Daniels, W. A. Sosna, E. R. Jeppesen, J. M. Owells, M. D. Halpern, K. S. McCurdy, J. O. Rayner, and J. A. Lednicky. 2010. Gas-permeable ethylene bags for the small scale cultivation of highly pathogenic avian influenza H5N1 and other viruses in embryonated chicken eggs. Virol J. 7:e23.
- Lednicky, J. A., and J. O. Rayner. 2006. Uncommon Respiratory Pathogens. Curr Opin Pulm Med. 12, 235-239.
- Tuttle, R. S., W. A. Sosna, D. E. Daniels, S. B. Hamilton, and J. A. Lednicky. 2010. Design, assembly, and validation of a nose-only inhalation exposure system for studies of aerosolized viable influenza H5N1 virus in ferrets. Virol J. 2010 7:e135.
- Cutrone, R., J. Lednicky, G. Dunn, P. Rizzo, M. Bocchetta, K. Chumakov, P. Minor, and M. Carbone M. “Some oral poliovirus vaccines were contaminated with infectious SV40 after 1961,” Cancer Res. 65:,10273-10279 (2005).
- Lednicky, J. A., J. Dubach, T. P. Meehan, M. J. Kinsel, M. Bochetta, L. L. Hungerford, N. A. Sarich, K. E. Witecki, M. D. Braid, C. Pedrak, and C. M. Houde, “Genetically distant American Canine distemper virus lineages have recently caused epizootics with somewhat different characteristics in raccoons living around a large suburban zoo in the USA,” Virol. J., 1, 2 (volume 1, article 2) (2004).
- Forsman, Z. H., J. A. Lednicky, G. E. Fox, R. C. Willson, Z. S. White, S. J. Halvorson, C. Wong, A. M. Lewis, Jr., and J. S. Butel, “Phylogenetic analysis of polyomavirus simian virus 40 from monkeys and humans reveals genetic variation,” J Virol., 78, 9306-9316 (2004).
- Zdziarski, J.M., N. A. Sarich, K. E. Witecki, and J. A. Lednicky, “Molecular Analysis of SV-40-CAL, a New Slow Growing SV-40 Strain from the Kidney of a Caged New World Monkey with Fatal Renal Disease,” Virus Genes, 29, 183-190 (2004).
- Wright, M. H., L. M. Cera, N. A. Sarich, and J. A. Lednicky, “Reverse Transcription—Polymerase Chain Reaction Detection and Nucleic Acid Sequence Confirmation of Reovirus Infection in Laboratory Mice with Discordant Serologic Indirect Immunofluorescence Assay and Enzyme-Linked Immunosorbent Assay Results,” Comparative Medicine, 54, 410-417 (2004).
- Rubinas, T. C., R. B. Carey, M. C. Kampert, S. Alkan, and J. A. Lednicky, “Fatal Hemorrhagic Pneumonia Concomitant with Chlamydia pneumoniae and Parainfluenza virus 4 Infection,” Archives of Pathology and Laboratory Medicine, 128, 640-644 (2004).
- Lednicky, J. A., T. P. Meehan, M. J. Kinsel, J. Dubach, L. L. Hungerford, N. A. Sarich, K. E. Witecki, M. D. Braid, C. Pedrak, and C. M. Houde, “Effective primary isolation of wild-type Canine distemper virus in MDCK, MV1 Lu and Vero cells without nucleotide sequence changes within the entire haemagglutinin protein gene and in subgenomic sections of the fusion and phospho protein genes,” Journal of Virological Methods, 118, 147-157 (2004).
- Ling, P. A., J. A. Lednicky, W. A. Keitel, David Poston, Z. S. White, R-S Peng, Z. Liu, S. K. Mehta, D. L. Pierson, C. M. Rooney, R. A. Vilchez, E. O’Brien Smith, and J. S. Butel, “The dynamics of herpesvirus and polyomavirus reactivation and shedding in healthy adults: a 14-month longitudinal study,” Journal of Infectious Diseases, 187, 1571 – 80 (2003).